In a dideoxy DNA sequencing experiment, four separate reactions a... | Study Prep in Pearson
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SOLVED: Dideoxy DNA sequencing is based on chain termination with dideoxynucleotides. Why are normal deoxynucleotides also included in the reaction?to provide a 2'-OH (hydroxyl) required for incorporation of the dideoxynucleotidesto allow production of range of synthesis products of different lengthsto act as primer for chain termination by the dideoxynucleotidesto provide a template for DNA polymerase
SOLVED: Dideoxy DNA sequencing is based on chain termination wit…
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