Fluorescein isothiocyanate - Wikipedia
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the
Fluorescein Isothiocyanate - an overview | ScienceDirect Topics
Fluorescein isothiocyanate (FITC) is a fluorescent probe that is commonly used in the preparation of conjugates with biological molecules. It has high water solubility, bright fluorescence, and low nonspecific binding with biological tissues.
Fluorescein isothiocyanate (FITC) - AAT Bioquest
FITC (fluorescein isothiocyanate) is widely used to label proteins, antibodies, peptides, hormones, amine-modified oligonucleotides, and other amine-containing molecules with the green fluorescent dye fluorescein.
Fluorescein (FITC) | Thermo Fisher Scientific - US
FITC is a green fluorescein derivative with excitation max. of 498 nm, emission max. of 517 nm and displays a high rate of photobleaching.
Fluorescein Isothiocyanate - an overview | ScienceDirect Topics
Fluorescein, often as fluorescein isothiocyanate (FITC), is used to label proteins and, in particular, antibodies. FITC-casein is a classical fluorogenic protease substrate; autoquenching occurs between different fluorescein chromophores coupled to the same casein molecule, but quenching is released by proteolysis to produce a fluorescence ...
Fluorescein isothiocyanate (FITC) Biological Tracer - MilliporeSigma
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl ...
Characterization and optimization of fluorescein isothiocyanate ...
Fluorescein isothiocyanate (FITC) is widely used to fluorescently label reactive lysine residues on proteins, including antibodies. The rate and extent of labeling varies with reaction conditions, concentration of label, and the concentration and nature of the protein.
Fluorescein Isothiocyanate - an overview | ScienceDirect Topics
An isothiocyanate derivative of fluorescein is synthesized by modification of its lower ring at the 5- or 6-carbon positions. The two resulting isomers are nearly identical in their reactivity and spectral properties, including excitation and emission wavelengths and intensities.
Fluorescein isothiocyanate (FITC) - Molecular Imaging and …
4-[2-(3,4,5,6-Tetrahydropyrimidin-2-ylamino)ethyloxy]benzoyl-2--[N-3-amino-neopenta-1-carbamyl)]-aminoethylsulfonylamino-β-alanine fluorescein thiourea ; Fluorescein-conjugated cyclic decapeptides, CGLIIQKNEC (CLT1) and CNAGESSKNC (CLT2)
Biomedical and Pharmacological Uses of Fluorescein Isothiocyanate ...
Fluorescein isothiocyanate-labeled chitosan-based NCs (FITC@ChNCs) are used extensively in biomedical and pharmacological applications. The main advantage of using FITC@ChNCs consists of the ability to track their fate both intra and extracellularly.
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