A big cat sanctuary in Washington is temporarily closed and quarantined after bird flu killed 20 wild cats there, including cougars, bobcats and a tiger.

Twenty big cats, including a half-Bengal tiger and four cougars, died between late November and mid-December at a sanctuary in Washington State after becoming infected with bird flu, according to the facility’s director.

Twenty big cats have died after contracting bird flu at a Washington State animal sanctuary. The Wild Felid Advocacy Center of Washington in the city of Shelton announced the deaths on December 20. Just two weeks prior, officials confirmed the presence of bird flu in the sanctuary, which forced the center to close and quarantine.