A compact RNA-guided enzyme, NovaIscB, derived from bacterial IscBs—evolutionary ancestors of Cas9—has been engineered for efficient, programmable genome editing in human cells.

Since Cas9 was discovered in 2012, many further developments have been made, including variants with even greater accuracy. For example, Cas9-HF1 (or “HiFi”) was designed to reduce non-specific ...

CRISPR-Cas9 genome editing has revolutionized the food and medicine industries. The method involves introducing Cas9 nuclease—an enzyme that cuts DNA—into cells, with a synthetic guide RNA ...

The emergence of CRISPR-Cas9 genome-editing tools created new opportunities for enhancing plant and crop traits in agricultural biotechnology research. Now, multiplex genome-editing (MGE) technologies ...

CRISPR-Cas9 combines a protein that can neatly snip DNA with a molecule that guides those molecular shears to a specific spot in an organism’s genome. Watch this video to learn how it works.

SuperFi-Cas9 is 4,000 times less likely to cut off-target sites but just as fast as naturally occurring Cas9. Jack Bravo, PhD, a postdoctoral fellow in the lab of David Taylor, ...

CRISPR-Cas9–mediated gene editing has the potential to permanently reverse pathologic DNA mutations. However, with the permanence of gene editing comes the overarching problem of terminating ...

The CRISPR/Cas9 system provides an easy way to edit specific site/s in the genome and thus offers tremendous opportunity for human gene therapy for a wide range of diseases. However, one major ...

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nature Communications , 2017; 8: 14500 DOI: 10.1038/ncomms14500 Cite This Page : ...

In order for Cas9 to cut a DNA target, it needs to be directed to the target site by what is called a guide RNA.The guide RNA contains the complementary sequence to the DNA target site, working like a ...

CRISPR-Cas9 evolved in bacteria as an immune system to protect against viral infections, but in the past decade it has excited both researchers and the general public as a general-use gene editing ...