2013) with an assay sensitivity of 0.04 ng/mL and intra-assay coefficient of variation of 8.2%. Adult GnRH-Cre,GCaMP6s mice were given a lethal overdose of pentobarbital (3mg/100μL, i.p.) and perfused ...

(8) with some major modifications. This assay was performed in 96-well round-bottom plates (Greiner Bio-One, #650101). Each well contained a final reaction mixture of 65 μl. First, 10 μl citrated ...

Intra-and inter assay coefficients of variations (CV) were 4.0% (n = 4) and 3.5% (n = 3), respectively, and average recovery was108% (range 98.4–118 %). All statistical analyses were performed using ...