Genes contain instructions for making proteins, and a central dogma of biology is that this information flows from DNA to RNA ...

ICE default parameters provided InDel patterns and their relative ratios, enabling alignment of the non-targeting control (NTC) trace sequence with a gene-specific sgRNA-edited trace ... studies ...

It allows the evaluation of the quality of gene editing experiments using targeted next generation sequencing (NGS) data (`targeted`) as well as the discovery of important genes from knock-out or ...

Although the authors findings are interesting, they do little to demonstrate new scientific information or advancements in producing genetically modified livestock with improved production ...

Copyright held by the owner/author(s). This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International ...

With advancements in gene editing technology, researchers are continually attempting to modify various mosquito genes and validate their functional roles with CRISPR-Cas9 technology. The authors ...

A new study in Heart Rhythm, the official journal of the Heart Rhythm Society, the Cardiac Electrophysiology Society, and the Pediatric & Congenital Electrophysiology Society, published by ...

For this simultaneous delivery of single guide RNAs (sgRNA), ribonucleoparticles (RNP) were generated by incubating the sgRNAs with the Streptococcus pyogenes Cas9 nuclease (Integrated DNA ...

Cas9 endonuclease and sgRNA are delivered to cells in a vector that is modified to be transcribed and subsequently packaged in trans by the infecting HIV virus. Deep sequencing of packaged HIV-CRISPR ...