parts of the samples in both media and cell lysates were transferred to 96-well plates for BCA assays (Pierce BCA assay kit, Thermo Fisher Scientific, Waltham, MA, USA) to quantify protein content.
Pierceâ„¢ BCA Protein Assay Kits (23,227), SuperSignal West Pico PLUS (34,580) and DNase I (18068015) were obtained from Thermo Fisher Scientific (Jiangsu, China). Dihydroethidium (DHE) (KGAF019, KeyGEN ...
The lysate was transferred to a new tube and frozen at -80°C for storage. The protein content was determined using Pierce Micro BCA protein assay (Thermo Fisher Scientific, 23227). Protein samples ...
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Urine supernatant protein concentration was determined using Pierce 660-nm Protein Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). Enzyme-linked Immunosorbent Assays for IL-8 ...
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