At the completion of the reaction, amplification products are analyzed by size fractionation using gel electrophoresis. Real-time PCR follows the same course, except that the products are detected ...

Endpoint analysis: Scientists analyze PCR products typically using agarose gel electrophoresis. Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount ...

All PCR products were resolved on a 1X TAE, 2% agarose gel. Image Credit: Norgen Biotek Corporation Figure 4. Specific Separation and Subsequent Amplification of Cytoplasmic and Nuclear Transcripts.

Clean the emulsified (Step 9) and nonemulsified (Step 6) PCR products using the Wizard SV Gel and PCR Clean-Up System. Problem: A coarse emulsion is formed. Low-quality emulsions can be identified ...

their gel electrophoresis results were also large smears. They needed to determine if their product was part of this smear. Over time, the team experienced a series of minor celebrations as they ...

Each gel will be shared by 2 groups on the same bench. 3. Each group will load PCR and control samples on one side of a central molecular weight markers lane containing 5 µl of 1 Kb ladder.