1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

The current most widely used mass spectrometry (MS)-based proteomic ... because of the effort required for establishing SRM assays for every protein. The initial selection of optimal proteotypic ...