Another common use of colorimetric assays includes total protein quantification. For example, the BCA assay is one that combines the reduction of Cu2+ into Cu1+ by proteins in an alkaline medium, with ...

The PamChips® are blocked in 2% Bovine Serum Albumin (BSA). Following protein concentration determination (by BCA assay), 5-10 μg of protein for the PTK chip (~1 μg for the STK) will be loaded per ...

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