The Morphology and Assembly of Respiratory Syncytial Virus
WEBAug 20, 2018 · In this study, we have established an optimized system for studying RSV structure by imaging RSV-infected cells on transmission electron microscopy (TEM) grids by cryo-electron tomography (cryo-ET). Our results demonstrate that RSV is filamentous across several virus strains and cell lines by cryo …
Architecture of respiratory syncytial virus revealed by electron ...
WEBJul 2, 2013 · We carried out an electron cryotomographic characterization of cell culture-grown human respiratory syncytial virus to determine the architecture of the virion. The particles ranged from 100 nm to 1,000 nm in diameter and were spherical, filamentous, or a combination of the two.
The Morphology and Assembly of Respiratory Syncytial Virus …
WEBAug 20, 2018 · In this study, we have established an optimized system for studying RSV structure by imaging RSV-infected cells on transmission electron microscopy (TEM) grids by cryo-electron tomography (cryo-ET).
Structural landscape of the respiratory syncytial virus ... - Nature
WEBSep 15, 2023 · This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic...
Electron microscopy of the human respiratory syncytial virus …
WEBMay 25, 2000 · Abstract. Full-length fusion (F) glycoprotein of human respiratory syncytial virus (HRSV) and a truncated anchorless mutant lacking the C-terminal 50 amino acids were expressed from vaccinia recombinants and purified by immunoaffinity chromatography and sucrose gradient centrifugation.
Respiratory Syncytial Virus (RSV) - PMC - National Center for ...
WEBOct 31, 2014 · Negative contrast electron micrograph of respiratory syncytial virus. The complete genome of RSV (A2 strain) has been sequenced (see Fig. 160-2 ). 12 , 14 The viral RNA consists of 15,222 nucleotides containing 10 genes.
The Morphology and Assembly of Respiratory Syncytial Virus
WEBThe Morphology and Assembly of Respiratory Syncytial Virus Revealed by Cryo-Electron Tomography. by. Zunlong Ke. 1,2,†, Rebecca S. Dillard. 1,‡, Tatiana Chirkova. 1, Fredrick Leon. 1, Christopher C. Stobart. 1,§, Cheri M. Hampton. 1,‖, Joshua D. Strauss. 1, Devi Rajan. 1, Christina A. Rostad. 1, Jeannette V. Taylor. 3, Hong Yi. 3, Raven Shah. 1,
Cryo-EM structure of the nucleocapsid-like assembly of respiratory ...
WEBAug 22, 2023 · Respiratory syncytial virus (RSV) is a nonsegmented, negative strand RNA virus that has caused severe lower respiratory tract infections of high mortality rates in infants and the elderly, yet...
Architecture of respiratory syncytial virus revealed by electron
WEBWe carried out an electron cryotomographic characterization of cell culture-grown human respiratory syncytial virus to determine the architecture of the virion. The particles ranged from 100 nm to 1,000 nm in diameter and were spherical, filamentous, or a …
Helical ordering of envelope-associated proteins and ... - PubMed
WEBDec 22, 2021 · Here, using cryogenic electron microscopy and tomography combined with computational image analysis and three-dimensional reconstruction, we show that there is extensive helical ordering of the envelope-associated proteins and glycoproteins of RSV filamentous virions.